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Nanjing Qinsi Technology Co., Ltd
ExoDisc Purification Disk
NegotiableUpdate on 05/10
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Overview
ExoDisc Purification Disk
Product Details
ExoDisc™ Purification discs are an ideal choice for rapidly, label free, and efficient separation of intact extracellular vesicles (EVs) from cell culture supernatants (CCS), urine samples, and small amounts of plasma samples. Enriched EVs with high yield and purity can prepare for downstream analysis, such as NTA（Nano particle tracking analysis technology）The SEM、TEM、 Protein blotting, ELISA, PCR, or sequencing are used to apply precision science to precision medicine.
Using urine samples, EVs from urine sources are a reliable source for real-time molecular characterization of disease states in precision medicine.
ExoDisc(Covering all applications of CCS, urine, and plasma)
Simple 3 steps [Time: 10-40 minutes]
application
Comparison with other EVs separation methods
Transfer ExoDisc ™ The performance was compared with two commonly used EV separation methods: ultracentrifugation (UC) and Exo spin separation ™。 Starting from 1ml of prostate cancer cell culture supernatant, EVs were isolated using three different protocols and characterized by nanoparticle tracking analysis (NTA), enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). ExoDisc ™ Compared with the ultracentrifugation method, the recovery rate of EVs is>95%, and the concentration of mRNA is>100 times.
Complete EVs
advantage
|
category |
ExoDisc |
UC |
Exo-spin |
|
EVsSeparation time(h) |
0.5 |
6 |
4 |
|
Maximum centrifugal force(g) |
500 |
150,000 |
16,000 |
|
NTA (109 grain/mL) |
25.2 ± 0.2 |
6.5 ± 0.1 |
9.1 ± 0.8 |
|
purity(107grain/μgprotein) |
50.5 ± 16.3 |
2.0 ±0.1 |
0.4 ±0.9 |
|
SandwichELISA CD9/CD81 EVsconcentration(108CD9-EVs/mL) |
120 ±0.1 |
9.1 ±2.5 |
23.5 ±1.9 |
|
totalRNA (ng/μL) |
46.3 ± 7.3 |
2.8 ± 2.3 |
20.7 ±4.1 |
|
mRNAexpression(WithUCCompared to folding changes) |
|
|
|
|
-GAPDH |
168.9±3.6x |
1x |
42.2 ±0.8x |
|
-CD9 |
157.6±16.2x |
1x |
45.3 ±3.7x |
|
-PSA |
111.4±6.1x |
1x |
27.9±0.5x |
|
-PSMA |
207.9 ±10.8x |
1x |
45.3 ±2.8x |
Comparison with other EVs separation methods
Transfer ExoDisc ™ The performance was compared with two commonly used EV separation methods: ultracentrifugation (UC) and Exo spin separation ™。 Transfected with AR-FL and AR-V7Human pancreatic cancer cells
EVs isolated from ATCC CRL-1435 were mixed into 4ml of urine. Characterization of isolated EVs was performed using nanoparticle tracking analysis (NTA), enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). ExoDisc ™ Compared with the ultracentrifugation method, the recovery rate of EVs is>95%, the yield is 3-17 times higher, and the purity is>5 times higher.
Comparison with ultracentrifugation method
Application: Monitoring tumor growth in a live mouse xenograft model
Application: Protein analysis of plasma EVs in cancer patients
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