SpectraMax i3x multifunctional enzyme-linked immunosorbent assay (ELISA) reader

The continuous spectrum four function (light absorption, fluorescence, chemiluminescence, fluorescence resonance energy transfer) upgradable detection platform launched by MD allows users to upgrade to new application modules, including time-resolved fluorescence, fluorescence polarization, upconversion luminescence, TR-FRET, HTRF, AlphaScreen, Western blot, etc. Users can also choose a syringe for rapid dynamic detection or the MiniMaxTM 300 cell imaging module for cell viability, protein expression, apoptosis and other detection.

The SpectraMax i3x multifunctional detection platform launched by Molecular Devices can meet the infinite possibilities of multifunctional detection systems as your future needs change.

SpectraMax ® In addition to the full wavelength light absorption, fluorescence, chemiluminescence, and FRET detection functions, the i3x multifunctional reading machine is also compatible with client-side modular upgrade function. Users can upgrade to fluorescence polarization FP at any time according to their needs HTRF、AlphaScreen、Western Blot、 Upconversion luminescence detection, cell imaging, and rapid kinetic detection with syringe mode.

The SpectraMax i3x system can help you unravel the mysteries of cellular pathways through scientific means, as well as detect and analyze the activity and expression of proteins in samples. One investment will benefit for life. The detection system purchased for the first time can flexibly add new detection functions according to your future needs without the assistance of maintenance engineers or considering the losses that may be caused by system downtime. With the unlimited expansion of your research field.

main features

● User side upgradable design for application modules (including imaging)

High sensitivity full spectrum detection using spectral fusion technology

Ultra wide dynamic detection range based on cold PMT technology

● Widely used software SoftMax Pro for control and data analysis

Customized special testing card box service

application area

Quantitative and purity detection of DNA, RNA, and proteins, as well as quantitative detection of nucleic acids and proteins using ultra microplates, ELISAs/enzymatic kinetics detection, ion channel detection, drug degradation experiments, cell viability, cytotoxicity, cell proliferation detection, Caspase-3/7 and protease detection, CatchPoint cAMP detection, receptor ligand binding, SNP genotyping, drug target research, tryptophan autofluorescence detection, green fluorescent protein detection, reporter gene detection, ADME Tox experiment, cell migration, kinase and ATPase analysis, bioluminescence fluorescence resonance energy transfer (BRET), Multi Tox cell viability detection, HTRF technology related experiments based on CisBio's patent in France, AlphaScreen technology related experiments, cell imaging Analysis, Western Blot, and Upconversion Luminescence (UCPs).

From imaging analysis of cell density and changes in cell viability under different processing conditions to quantitative analysis of nucleic acids and proteins using Western Blot method, all requirements can be met with just one instrument. And it is equipped with powerful and flexible software, which integrates data collection and analysis. From raw data to the final published results, only one detection system is needed to comprehensively analyze various indicators of cells and proteins to be detected.

Experimental examples

As is well known, prolonged exposure of normal cells to high temperatures can cause cell apoptosis and also increase the expression level of HSP70 (Heat Shock Protein) in cells. We placed CHO-K1 cells in two different growth environments, namely a healthy environment and a stress environment. Firstly, the cells in both environments were subjected to heat shock treatment, and the cell imaging function of this multifunctional detection platform was used to detect their cell proliferation, vitality, and apoptosis. At the same time, we could also use the ScanLaterTM Western Blot detection function to obtain data. After analysis, it was found that the expression level of HSP70 was positively correlated with the heat shock response.