SpectraMax Plus 384 Light Absorption ELISA reader

Molecular Devices launches SpectraMax ® The Plus 384 now simultaneously implements the functions of a standard spectrophotometer and a microplate reader. It can read samples from any standard specification colorimetric dish, 12x75 mm test tube, 96 or 384 well microplate. The host is equipped with a high-precision grating monochromator, which can detect UV/visible/near-infrared light without the need for filters. It can be freely selected between 190nm and 1000nm, with high-precision temperature control function, up to 45 ° C, meeting the experimental applications that require temperature control, SpectraMax ® The Plus 384 adopts a multi-channel detection mode, which can work like a dual wavelength spectrophotometer. It consists of 9 independent sample detectors and a reference detector, which reduces all optical path output errors caused by optical fibers and increases detection speed. The 96 well plate only takes a minimum of 5 seconds. The patented non temperature dependent pathcheck optical path sensor technology can automatically correct the measured absorbance value in the microplate to the absorbance value under the 1cm cuvette optical path, making the reading of the microplate reach the accuracy of the spectrophotometer. The calibration result does not change with temperature changes. The instrument can work with StakMax ® Stacking machine or third-party automation equipment integration compatibility.

main features

Ultra wide wavelength coverage of 190-1000 nm, 1 nm step

The bandwidth is only 2 nm

● Detection range 0-4 OD

● Supports 96 or 384 type microplates

● Endpoint method, kinetic method, spectral scanning method

Patent Pathcheck optical path correction function

● Equipped with a standard 1 cm vertical colorimetric dish slot

Accurate temperature control and automatic oscillation function

SpectraDrop ultra micro detection module, only requires 2 uL, maximum throughput of 64 samples/time, dsDNA detection sensitivity of 2 ng/uL

● Professional grade SoftMax Pro software, integrating instrument control and data analysis

application area

It can mainly be used for rapid quantitative detection of DNA, RNA, and proteins, and is widely used in ELISA/EIA detection, microbial growth/MIC, IC50/LD50, cytotoxicity/cell proliferation, bacterial endotoxin detection, reporter gene detection, cytokine detection, and has a kinetic detection mode. It can also be used for enzymatic detection and kinetic ELISA detection.

Experimental examples

The SpectraMax Plus 384 simultaneously realizes the functions of a standard spectrophotometer and a microplate reader. It is an ideal device in the field of life science research. It can read samples from any standard specification colorimetric dish or 12x75 mm test tube, 96 or 384 well microplate, and the detection wavelength range covers the ultraviolet region, visible region, and near-infrared air. Because according to Beer's law, the nucleic acid concentration corresponding to 1 OD at a standard 1cm optical path is constant, but the microplate cannot reach a 1cm optical path due to volume limitations. With the help of an independent vertical colorimetric dish slot, it can reliably and quickly quantify nucleic acid and protein concentrations without opening a computer.