SpectraMax Gemini XPS fluorescence enzyme-linked immunosorbent assay (ELISA) reader

SpectraMax Gemini ® XPS is a full wavelength fluorescence enzyme-linked immunosorbent assay (ELISA) developed by Molecular Devices, which can quickly determine the optimal excitation and emission wavelengths and can be applied under flexible experimental conditions in most fluorescence intensity experiments. SpectraMax Gemini ® XPS adopts a dual grating continuous spectrum design, which can detect various novel fluorescent dyes without the need to purchase expensive filters, SpectraMax Gemini ® The XPS top adopts a patented 12 degree fluorescence angle design, which can effectively avoid the interference of excitation light on emission light and improve the detection signal-to-noise ratio. Auto PMT (photomultiplier tube automatic gain adjustment technology) can effectively expand the range of detection dynamics, avoiding the occurrence of photobleaching and fluorescence supersaturation phenomena. In addition, the instrument is equipped with two secondary detection modes, chemiluminescence and time-resolved fluorescence, which facilitate users to expand to more detection methods under limited resources.

main features

EX 250-850 nm, EM 360-850nm, 1nm step size

● Supports 6-384 well plates

Long life high-energy xenon flash lamp

● Ultra wide range of dynamic testing

Endpoint method, kinetic method, spectral scanning method, pore domain scanning method

The patented 12 degree fluorescence angle design effectively avoids excitation light interference and improves signal-to-noise ratio

● Secondary detection mode (time-resolved fluorescence, chemiluminescence)

● Professional grade SoftMax Pro software, integrating instrument control and data analysis

application area

Cell penetration assay, cell proliferation, cell activity, cytotoxicity detection, nucleic acid quantification detection, green fluorescent protein detection, phosphatase/kinase detection, microbial growth detection, NanoOrange protein quantification, protease fluorescence detection, cell apoptosis, CatchPoint cAMP/cGMP detection, etc.

Experimental examples

We know that more sensitive methods are needed for next-generation sequencing and amplification product quantification. Life Technologies' Quant iT PicoGreen dsDNA experiment has better specificity for DNA and is 1000 times more sensitive than traditional absorption light methods. To maximize the sensitivity of the experiment, it is necessary to use the optimal excitation and emission wavelengths. The SpectraMax XPS multifunctional microplate reader, unlike color filter based readers, does not have dual gratings that allow users to select any wavelength within the instrument's nominal range. It is important to determine the excitation and emission wavelengths that can generate the optimal experimental dynamic range. The sensitivity observed using the 96 well microplate format is as low as 63 pg/mL, which is significantly lower than the nominal 250 pg/mL in the QuantiT PicoGreen experimental product manual.