SpectraMax 190 light absorption enzyme-linked immunosorbent assay (ELISA) reader

SpectraMax ® 190 is a full wavelength light absorption enzyme-linked immunosorbent assay (ELISA) reader launched by Molecular Devices, which has a super high cost performance ratio. It is specifically designed to optimize the detection light path for 96 well microporous plates. It has a built-in high-precision grating monochromator, which can be selected freely between 190nm and 850nm, and a minimum bandwidth of 2nm to ensure high accuracy in detection; High precision temperature control function, temperature control range from room temperature+4 ° C to 45 ° C, and can also perform linear oscillation.

SpectraMax ® 190 adopts a multi-channel detection mode, which can work like a dual wavelength spectrophotometer, consisting of 8 independent sample detectors and reference detectors, reducing all optical path output errors caused by optical fibers and increasing detection speed; The patented non temperature dependent pathcheck optical path sensor technology can automatically correct the absorbance value detected in the measured microplate to the absorbance value under the 1cm colorimetric dish optical path, enabling the reading of the microplate to reach the accuracy of a spectrophotometer. The calibration result does not change with temperature changes. The professional level SoftMax Pro software can automatically perform data calculation and storage, complete chart curve production, 19 curve fitting methods, and users can edit formulas and programs by themselves.

main features

UV/visible light range 190-850nm, 1nm step

Single hole can detect up to 6 wavelengths

● Endpoint method, kinetic method, spectral scanning method

Patent Pathcheck optical path correction function

Accurate temperature control and automatic oscillation function

SpectraDrop ultra micro detection module, only requires 2uL, with a maximum throughput of 64 samples/time, and a dsDNA detection sensitivity of 2ng/uL

● Support StakMax ® Fully automatic stacking machine

application area

It can mainly be used for rapid quantitative detection of DNA, RNA, and proteins, and is widely used in ELISA/EIA detection, microbial growth/MIC, IC50/LD50, cytotoxicity/cell proliferation, bacterial endotoxin detection, reporter gene detection, cytokine detection, and has a kinetic detection mode. It can also be used for enzymatic detection and kinetic ELISA detection.

Experimental examples

The SpectraMax 190 adopts a patented PathCheck sensor, which is an advanced patented technology for correcting the liquid level height (optical path) of samples, and the correction results are not affected by temperature changes. It can automatically convert the sample height in the microplate into a 1cm colorimetric cup optical path, eliminate the differences in liquid levels in the microplate, expand the range of dynamic analysis, and detect liquid addition errors. By using the full spectrum scanning mode, you can monitor the spectral detection of the sample throughout the process and select the most ideal wavelength for reading.