Compared to traditionalBiological Window Imaging, Near Infrared Second Zone(NIR II, 1000～1700 nm)Biological imaging has become a key direction of current scientific research, with visible light(400～750 nm)Near infrared region one(NIR I, 750～900 nm)Fluorescence imaging technology,Due toemission wavelengthshort,Cannot effectively suppressScattering of photons within biological tissues,The wavelength of near-infrared emission in the second region is longer, which canEnhance the light absorption of biological tissues,Make penetration possibleofDeeper Depth,Having higher temporal and spatial resolution.

Short wave infrared cameras900-1700nmWith excellent detection performance, various sensitivity shortwave cameras can be widely used in various near-infrared two zone imaging applications.

Picture1-Schematic diagram of near-infrared two zone macroscopic live imaging

Picture2-Intravenous injection of near-infrared two zone fluorescent dye

As shown in the above figure, inject near-infrared two zone fluorescent dye into the mouse body and select an appropriate optical imaging lens. Although the wavelength of near-infrared emission in the second region is longer, it canEnhance the light absorption of biological tissues,Make penetration possibleofDeeper Depth,However, the emitted fluorescence is still at a low light level and requires imaging detection using a deep cooled shortwave infrared camera. Picture2The photos taken were taken usingLD-SW6401725-80-UTake photos with a camera.

In near-infraredDriven by the advancement of bioimaging technology in Zone II, near-infrared fluorescence probe materials in Zone II have also experienced rapid development. Appeared likeCarbon nanotubes, fluorescent quantum dots, rare earthsmetalExcellent fluorescence properties exhibited by conjugated polymers, small molecules, etc. in the near-infrared regionThe materials. They can be roughly divided into three categories: inorganic nanomaterials, conjugated polymer based materialsNIR-Zone II dyes, based on organic small moleculesNIR-Zone II dye; These materials not only provide imaging conditions for near-infrared two zone biological imaging, but also have great application prospects in photothermal and photodynamic therapy, drug delivery, and surgical procedures.

In the preliminary research on the preparation of near-infrared two zone fluorescent probes, deep cooled shortwave infrared cameras also played a significant role. Most of these fluorescent probe materials have a wavelength range of1200mmAbove, conventional cameras cannot detect the luminescence of reagents. The following figure shows the adoption ofLD-SW6401725-80-UTake near-infrared fluorescence reagent images in the second zone.