Polarity embedding reverse phase chromatography;
Strong alkaline substances such as alkaloids can be analyzed under pH<7 conditions without tailing phenomenon;
Provide strong hydrogen bonding, suitable for complex component analysis, such as traditional Chinese medicine extracts;
Optimized the size of pore size and specific surface area, resulting in better pollution resistance;
Unique bonding technology ensures stable retention performance.
Venusil®HLP C18 chromatographic column in the previous generation Venusil®On the basis of HLP, the pore size and specific surface area of silica gel have been optimized, and more advanced bonding technology has been adopted to ensure its excellent reverse phase chromatography separation ability and unique selectivity. At the same time, it has better anti pollution ability when separating complex component samples.
Venusil®The unique feature of the bonding phase of HLP C18 chromatography column is the insertion of amide groups at the root of the traditional C18 stationary phase long chain. This "polar embedding group" can form hydrogen bonds with the silanol groups on the surface of silica gel or water in the mobile phase, providing a protective effect on the silica gel matrix and reducing the interaction between alkaline compounds and silanol groups, ensuring good peak shape of alkaline compounds.
This unique bonding technology ensures that alkaline compounds with complex structures exhibit separation performance different from traditional reverse phase chromatography, that is, good chromatographic retention of alkaline substances can be achieved without the need to use a mobile phase with pH>7 to inhibit the dissociation of alkaline substances; For complex component analysis, such as compounds with polarity, neutrality, and non polarity in a mixture, a single separation can be achieved with excellent anti pollution ability; In daily experiments, the bonding phase loss rate is extremely low and the retention performance is stable.
Silicone purity:>99.999% Particle size: 5 μ m Pore size: 110 Å Specific surface area: 340 m ²/g Carbon content 15% pH: 2.0-8.0*
Sample:1 mg/mL Amphetamine Flow Rate: 1 mL/min
Mobile phase:Methanol: 0.02M potassium phosphate buffer solution (0.1% triethylamine pH=2)=15:85
Injection volume:10 μ L detection wavelength: UV215 nm;
