Selection of Packing Materials for Solid Phase Extraction Columns
Firstly, based on the differences between the target compound and the interfering substance, such as polarity, molecular weight, pka value, etc., select the appropriate filler.
Selection of Solid Phase Extraction Column Specifications
For reverse phase, normal phase, and adsorption solid phase extraction columns, the mass of the extracted sample should not exceed 5% of the SPE column packing (the selectivity of the same adsorbent for different target substances varies. This value is for reference only!); For ion exchange solid-phase extraction columns, the capacity of ion exchange must be considered. The adsorption capacity of Cleanert SAX and SCX columns is approximately 0.2 meq/g. The following table shows the capacity and elution parameters of SPE columns:
specifications |
Maximum sample size |
volume |
Minimum elution volume |
50 mg-100 mg |
2.5 mg-5 mg |
1 mL |
100-200 μL |
100 mg-200 mg |
5 mg-10 mg |
3 mL |
200-500 μL |
200 mg-1 g |
10 mg-50 mg |
6 mL |
500 μL-6 mL |
500 mg-2 g |
25 mg-100 mg |
12 mL |
3 mL-10 mL |
2 g-5 g |
100 mg-250 mg |
25 mL |
10 mL-50 mL |
5 g-10 g |
250 mg-500 mg |
60 mL |
20 mL-50 mL |
