Programmable quantitative sealing machine

Name: Programmable Quantitative Sealing Machine

model: HM-FKJ

summary

Hengmei Technology's programmable quantitative sealing machine is driven by an intelligent circuit and has a dedicated heating roller inside. It is specifically designed for sealing 51 well and 97 well quantitative disks, and is used in conjunction with microbial detection reagents to provide a simple, fast, and accurate experimental plan for quantitative detection of total coliforms, Escherichia coli, fecal (heat-resistant) coliforms, enterococci, and Pseudomonas aeruginosa; The 51 well quantitative disk and 97 well quantitative disk are semi-automatic quantitative methods designed based on the traditional Maximum Possible Number (MPN) statistical model, which can quantitatively detect water sample results. This method can replace and outperform traditional (membrane filtration, and multi tube fermentation) methods for detecting microorganisms in water.

Scope of application

Used for rapid detection of Pseudomonas aeruginosa, Enterococcus, total Escherichia coli, fecal Escherichia coli, Escherichia coli, and total bacterial count in water samples, portable in the field, emergency, and quantitative testing.

Product Description

The enzyme substrate detection device consists of four parts: a programmable quantitative sealing machine, a 51 or 97 well quantitative detection disk, a 100mL quantitative bottle, and an enzyme substrate detection reagent. The detection water sample range includes drinking water, source water, bottled water, reclaimed water, secondary water supply, pipeline water, wastewater, food water, livestock water, medical water, etc.

Advantages and Characteristics

The enzyme substrate method is a standard method for detecting Escherichia coli included in GB5750-2006. It is currently the most advanced method for detecting Escherichia coli in water, and is gradually being recognized by domestic testing departments for its convenience, speed, low false positives, and suitability for rapid detection of large amounts of samples.

Compared with multi tube fermentation and membrane filtration methods, enzyme substrate method greatly reduces the detection steps and does not require high experimental environment. The detection time can be reduced to 24 hours, which has good application prospects in daily water sample monitoring and emergency monitoring. It can detect and prevent in a timely manner, and minimize the probability of major public safety accidents. Taking the total coliform group determination in GB5750-2006 as an example, three methods are compared, as shown in the table.

Table 1 Comparison and statistics of multi tube fermentation method, membrane filtration method, and enzyme substrate method

Method advantages:
1. No need to operate in a sterile room.
2. The manual operation time is less than 1 minute.
3. No need for culture medium preparation and extensive sterilization of glassware.
Qualitative and quantitative analysis can be completed in 4.24 hours without the need for validation experiments.
MMO-MUG culture medium using fixed substrate technology and enzyme substrate method in accordance with GB5750-2006 "Standard Test Methods for Drinking Water" is paired with a programmable quantitative sealing machine, and the water sample is packaged with (51 well quantitative disk) or (97 well quantitative disk) for detection.

Add a water sample containing total coliform bacteria, and cultivate the target bacteria in Minimum Medium ONPG-MUG medium at 36 ℃± 1 ℃. The specific biological enzyme β - galactosidase produced by total coliform bacteria can decompose the chromogenic substrate ONPG in Minimum Medium ONPG-MUG medium, making the culture appear yellow; At the same time, Escherichia coli in the water sample produces specific β - glucuronidase to decompose the fluorescent substrate MUG in the Minimum Medium ONPG-MUG medium, producing characteristic fluorescence. Similarly, the heat-resistant coliform bacteria (fecal coliform bacteria) will decompose the chromogenic substrate ONPG in the Minimum Medium ONPG-MUG medium when cultured at 44.5 ℃, resulting in a yellow color of the medium.

technical parameter

to configure

• Previous: No more
• Next article: No more