Nucleic acid extraction and purification kit

Nucleic acid extraction and purification kitBefore using nucleic acid extraction reagents:

Transfer the proteinase K solvent into the lyophilized powder containing proteinase K and mix well.

Swab extraction steps:

Add 0.6ml CY1 liquid and 10ul proteinase K to dry swab collection, mix well, and incubate in an air incubator at 65 ℃ for 30 minutes (or wet collection: centrifuge the sample centrifuge tube containing swab and preservation solution at 12000 rpm for 1 minute, retain the precipitate, and remove the supernatant). Add 0.6ml CY1 liquid and 10ul proteinase K, mix well, and incubate in an air incubator at 65 ℃ for 30 minutes.

Remove the swab and centrifuge at 1200rpm for 1 minute.

Take out all the supernatant and transfer it to a new centrifuge tube for experimentation.

Add 0.25ml of CY-2 liquid and 10ul of magnetic beads (shake well before use), mix well for 12 minutes, place on a magnetic rack and let it stand for 30 seconds, then remove the liquid.

Add 0.6ml CY-3 liquid, mix well for 3 minutes, place it on a magnetic rack and let it stand for 30 seconds, then suck off the liquid.

Add 0.6ml of CY4 liquid, mix well for 3 minutes, place on a magnetic rack and let it stand for 30 seconds, then suck off the liquid.

Repeat step 2.6

Dry at room temperature for 10-20 minutes and wash with 50 ul of CY5 liquid. Mix well, place on a magnetic rack and let it stand for 30 seconds, then transfer the liquid to a new centrifuge tube.

Measuring OD

Saliva extraction steps:

Mix saliva and preservation solution in a 1200rpm centrifuge tube for 1 minute;

Retain the sediment and remove the supernatant;

Add 0.6ml of CY1 liquid and 10ul of proteinase K into it, mix well, and incubate in an air incubator at 65 ℃ for 30 minutes;

Centrifuge at 1200rpm for 1 minute, take out all the supernatant and transfer it to a new centrifuge tube. Add 10ul magnetic beads and 0.25ml CY2, mix well for 12 minutes, and place it on a magnetic rack for 30 seconds to remove the liquid.

Add 0.6ml of CY3 liquid, mix well for 3 minutes, place it on a magnetic rack and let it stand for 30 seconds, then suck off the liquid.

Add 0.6ml of CY4 liquid, mix well for 3 minutes, place on a magnetic rack and let it stand for 30 seconds, then suck off the liquid.

Repeat step ⑥

Dry at room temperature for 10-20 minutes, add 50 ul of CY5 liquid for elution, mix well, place on a magnetic rack for 30 seconds, and transfer the liquid to a new centrifuge tube

Measuring OD

Note: If RNA removal is required, RNaseA10mg/mL can be prepared in a solvent (10mm sodium acetate: pH 5.0), boiled for 15 minutes, adjusted to pH 7.5 with Tris HCl, and stored at -20 ℃.

Nucleic acid extraction and purification kitPrecautions for using nucleic acid extraction or purification reagents:

This product is only used for in vitro diagnostics.

The storage environment and extraction steps should strictly follow the instructions in the manual.

If a small amount is found during extraction, the sample size can be appropriately increased or the number of extractions can be increased.

The extracted DNA must be kept fresh and tested in a timely manner.

Note: This extraction reagent is used for in vitro diagnosis and cannot be used for oral or external administration in humans or animals. If swallowed, it can lead to serious incidents; Has a certain degree of irritation to the eyes and skin. If accidentally splashed into the eyes, rinse with water. Ventilation should be maintained during use.