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Beijing Hongzuo Shengwei Technology Co., Ltd
LIFD230 Laser Induced Fluorescence Detector
NegotiableUpdate on 03/19
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Overview
Laser induced fluorescence detector (LIFD) is currently one of the most sensitive detectors used for detecting chemical and biological samples, widely used in separation fields such as high performance liquid chromatography (HPLC), micro column liquid chromatography (Micro LC), and capillary electrophoresis (CE). Especially in the single-molecule detection of ultra trace bioactive substances, the determination of ultra trace bioactive substances and environmental pollutants in biological samples is widely used, such as capillary
Product Details
Technical parameters:
Relative Fluorescence Unit (RFU) 0-1000
Dynamic range (within 1000 RFU range)>104
Sensitivity (mol FITC/L)>1012
Baseline noise (RFU)<0.005
Baseline drift (RFU/h)<0.2
Excitation wavelength (nm) 473
Emission center wavelength (nm) 525 (510-540)
Laser type 10mW DPSS laser 473nm
Filter type>510nm long pass filter
Two color mirror with passband>490nm
510-540nm bandpass filter
Power supply requirements: AC, 110/220V, 50/60Hz
Working temperature range (℃) 15-35
Relative humidity<75%
Detection pool Z-type flow aperture 0.8mm, detection optical path 6.8mm, pool volume 3.42 μ L
Relative Fluorescence Unit (RFU) 0-1000
Dynamic range (within 1000 RFU range)>104
Sensitivity (mol FITC/L)>1012
Baseline noise (RFU)<0.005
Baseline drift (RFU/h)<0.2
Excitation wavelength (nm) 473
Emission center wavelength (nm) 525 (510-540)
Laser type 10mW DPSS laser 473nm
Filter type>510nm long pass filter
Two color mirror with passband>490nm
510-540nm bandpass filter
Power supply requirements: AC, 110/220V, 50/60Hz
Working temperature range (℃) 15-35
Relative humidity<75%
Detection pool Z-type flow aperture 0.8mm, detection optical path 6.8mm, pool volume 3.42 μ L
Main features:
■ Brand new optical path system module
Using a semiconductor diode laser as the excitation light source and a confocal optical configuration, the background noise caused by Rayleigh scattering and Raman scattering is reduced;
Using a combination of long pass and band-pass filters on the emission path to reduce laser scattering entering the photomultiplier tube and improve the signal-to-noise ratio of the detector;
Optimize the numerical aperture of the focusing objective lens to match its working distance with its focusing ability, and use a large numerical aperture objective lens to increase its lighting efficiency.
■ Specialized detection pool module
Adopting a reflective Z-type detection cell, efficient fluorescence collection has been achieved, greatly improving the sensitivity of LIFD.
Specialized laser power module
Using high-frequency switching circuits to complete AC/DC conversion and precision integrated circuit feedback control.
Three terminal voltage regulator integrated chip, providing stable and reliable power supply for the entire circuit system; The matching laser power supply provides stable current for the laser.
■ Brand new optical path system module
Using a semiconductor diode laser as the excitation light source and a confocal optical configuration, the background noise caused by Rayleigh scattering and Raman scattering is reduced;
Using a combination of long pass and band-pass filters on the emission path to reduce laser scattering entering the photomultiplier tube and improve the signal-to-noise ratio of the detector;
Optimize the numerical aperture of the focusing objective lens to match its working distance with its focusing ability, and use a large numerical aperture objective lens to increase its lighting efficiency.
■ Specialized detection pool module
Adopting a reflective Z-type detection cell, efficient fluorescence collection has been achieved, greatly improving the sensitivity of LIFD.
Specialized laser power module
Using high-frequency switching circuits to complete AC/DC conversion and precision integrated circuit feedback control.
Three terminal voltage regulator integrated chip, providing stable and reliable power supply for the entire circuit system; The matching laser power supply provides stable current for the laser.
