Constant high-pressure crusher

Principle of fragmentation
The Constant Systems high-pressure cell disruptor is specifically developed for biotechnology users, taking into account the characteristics of biological samples in its design. It has unique designs for sample cooling, CIP, and SIP during fragmentation.
principle
The principle of Constant Systems high-pressure disruptor is to use high-pressure extrusion to squeeze the sample at high speed through a fixed small nozzle, and then impact it onto the impact target to break the cells.


The high-pressure piston moves downwards, and due to gravity, the sample is sucked into the high-pressure sleeve
Once the sample enters the high-pressure chamber, the piston accelerates at high speed to close the inlet valve and make the sample high
By quickly passing through the fixed nozzle, the sample pressure can reach 40kpsi (2700 bar), and the sample can quickly pass through
Transferring from the high-pressure zone to the low-pressure zone leads to cell fragmentation
The sample hits the impact target, flows through the cooling surface, and the cooled sample passes through due to gravity
Export out, enter the lower cycle


temperature control
Using a cooling jacket to cool the sample during the crushing process can effectively control the temperature of the crushed sample, thereby avoiding deactivation of the active substance.










reproducibility
Constant Systems fluid control system precisely controls the crushing pressure, ensuring the reproducibility and good crushing effect of the instrument.









characteristic
Constant Systems can generate pressures up to 40Kpsi (2700 bar) with excellent crushing efficiency, achieving very high crushing efficiency in one crushing operation, avoiding repeated crushing that can cause fragments to become too fine and cause denaturation and deactivation of active substances.
Constant Systems' crushing head has a cooling jacket design, which can effectively control the temperature of the crushed sample; To ensure timely cooling of the sample during the crushing process, thereby avoiding deactivation of the active substance due to temperature rise in the crushed sample; In addition, the injection storage tank can also be equipped with a jacket for sample pre cooling.
Constant Systems adopts a fixed nozzle design with good stability; Overcoming the disadvantages of valve design relying on regulating valves to adjust crushing pressure, such as poor pressure stability, poor reproducibility, and easy valve failure during the crushing process.
Constant Systems uses a hydraulic system to generate high pressure, which is stable and has good reproducibility.
Constant Systems high-pressure cell disruptor has a dead volume of less than 1ml (minimum less than 0.1ml) and no minimum sample processing capacity limit.
Constant Systems can handle sample concentrations up to 30% bacterial concentration, with high concentrations of broken sample products, making downstream separation and purification operations convenient; Can handle filamentous bacteria.
Constant Systems TS, B, and C series optional peristaltic pumps are used for fully automatic injection and fully enclosed operation, avoiding environmental impact or being affected by the environment.
Simple operation and maintenance, CIP and SIP are very convenient Choose specific CIP and SIP designs that comply with GMP and FDA standards.
Digital display of operating pressure and sample temperature, and optional data acquisition package to display and record the operating pressure and sample temperature of the entire crushing process, providing useful information for GMP.

application
Constant Systems cell disruptors have a wide range of applications, including cell lysis, protein and DNA extraction, enzyme release, DNA manipulation for selective fragmentation, single-cell separation, tissue fragmentation, etc. For example:

algae	Break various collar algae
Animal cells	Choose crushed chicken sperm cells
microorganism	Crushing recombinant Escherichia coli to obtain highly active enzymes; Crushing Bacillus subtilis achieves high protein recovery rate
Fungi (including yeast)	Beer yeast obtains 99% protein release, Pichia pastoris obtains 99% recombinant protein release, and Aspergillus obtains enzymes with high specific activity
Mammalian cells and tissues	Crushing horse liver cells and endoplasmic reticulum membrane, isolating single live cells from bovine spleen and lymph nodes
insect	Cytomegalovirus in fragmented human cell nuclei
Plant cells and tissues	Extracting banana pectin and strawberry leaf DNA
virus	Separate the membrane antigen of Toxoplasma gondii, separate the nucleus and membrane of amniotic cells
Environmental samples	Extracting DNA from soil microorganisms