Bole CFX Connect fluorescence quantitative PCR instrument

NegotiableUpdate on 04/08

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

The product features of the Bole CFX Connect fluorescence quantitative PCR instrument include the commonly used dual channel detection capability and FRET channel. The excellent optical path design does not require calibration with reference fluorescent dyes. Combined with the CFX Manager Software 3.0 software, it supports absolute quantification, relative quantification, probe allele typing, and high-resolution melting curve analysis, making it a cost-effective high-performance quantitative PCR instrument.

Product Details

Bole CFX Connect fluorescence quantitative PCR instrumentHaving commonly used dual channel detection capabilities andFRETChannel, excellent optical path design without reference fluorescent dye calibration, compatible withCFX Manager Software 3.0The software supports absolute quantification, relative quantification, probe allele typing, and high-resolution melting curve analysis, making it a cost-effective high-performance quantitative toolPCRInstrument.

Bole CFX Connect fluorescence quantitative PCR instrumentProduct parameters:

model	CFX Connect
Base	CFX Connect
ramp rate	5℃/s
Heating/cooling technology	Peltier
temperature range	0-100℃
Temperature accuracy	±0.2? C
Temperature uniformity	±0.4? C (90 ° C reached in 10 seconds)
Temperature gradient range	30–100? C
Temperature gradient temperature difference range	1–24? C
excite	3 LEDs with filters
detection	3 photodiodes with filters
Excitation/detection wavelength range	450–580 nm
Melting curve resolution	0.1? C
Multi detection capability	2 more genes per well
sensitivity	1 copy of human genomic DNA
dynamic range	10 orders of magnitude
sample capacity	96
reaction volume	1-50 µ l (recommended 10-25 µ l)
Data analysis mode	Absolute quantification of standard curve, melting curve analysis, high-resolution melting curve, allele typing, endpoint fluorescence analysis?? Cq gene expression analysis, none Limited data file merging analysis, suitable internal reference screening, relative quantification of multiple internal reference genes with amplification efficiency correction (bar chart, scatter plot, volcano plot, cluster analysis, heatmap).


data export	Excel ， Word or PowerPoint user reports contain runtime settings, graphical and tabular data results, and can be printed or saved directly in PDF format; Can be connected LIMS system.