Bio Rad C1000 PCR instrument

the United StatesBio Rad C1000 PCR instrumentTechnical Specifications:

96 blue-green LED sequentially excited samples greatly reduced fluorescence crossover

Suitable for 96 × 0.2ml reaction tubes or 12 0.2ml 8-linked reaction tubes.

It can support multiplex PCR, which not only saves reagents and time, but also makes it possible to use internal controls, thereby further controlling the efficiency of sample PCR.

■ * Temperature control performance and gradient PCR function.

The Opticon Monitor analysis software can easily set up RT-PCR and multiplex analysis

PCR and other reactions, as well as functional analysis such as DNA quantification, gene expression, and allele analysis.

Accurate and reliable PCR instrument:

Opticon's C100 PCR instrument is based on the mature Bio Rad product PTC-200, and its features include

Semiconductor refrigeration technology;

Three temperature sensors, dual zone temperature control;

Bio Rad C1000 PCR instrument from the United States

Accurate temperature control: within 30 seconds at 90 ℃, the accuracy is ± 0.3 ℃, even considering PCR reaction

The influence of liquid volume on temperature changes;

Good uniformity: Within 30 seconds at 90 ℃, the uniformity is ± 0.4 ℃;

96 reaction sample sizes;

Fast temperature rise and fall speed: up to 3.0 degrees per second;

There is a calculated temperature control mode: during the temperature rise and fall process, it does not rush over to ensure the accuracy of the experiment

Repeatability;

Temperature gradient performance: The sample module can have 12 different temperatures simultaneously from left to right,Create a temperature gradient, with the gradient temperature difference set to a minimum of 1 ℃ and a maximum of 24 ℃. By once

The experiment can determine the optimal annealing temperature or denaturation temperature, which not only saves time but also reduces the temperature

Low cost.

the United States

Fluorescence detection system combining dynamic and static detection:

Four independent excitation channels use light sources of different wavelengths (light-emitting diodes, LEDs)

）Excitation is carried out to achieve the highest excitation efficiency for each channel;

Four independent detection channels use detectors with different photosensitive ranges (photodiodes)

， PDT is used for detection to achieve the highest emission detection efficiency for each channel;

The light source does not generate heat, has a long service life, and does not need to be replaced for life;

The position of the light source and detector is relatively fixed, ensuring complete optical path and no holes between them

Interference issues;

The shortest optical path lengthQuantitative PCR instrumentAll optical paths are less than 8cm, resulting in minimal optical propagation loss

Four channel detection includes FAM, SYBR Green I, VIC, HEX, Joe

More than 10 fluorescent dyes including TAMRA, Cy3, Cy5, Texas Red, Rox, etc.

Wavelength range: 450-730nm;

Wide linear range: up to 10 orders of magnitude, capable of detecting single copy templates;

Excellent resolution: can completely distinguish between 32, 64, 128, 256, 1024 copies;