Aseptic Laboratory (Aseptic Room)

Adapt to the industry:Microbiology biomedical science. Biochemical animal experiments Suggestion level for restructuring: Level 100 to 100000

Design, construction and construction of sterile laboratory

Aseptic laboratory design

The sterile laboratory consists of six solution parts: preparation room, strip washing room, sterilization room, sterile room, constant temperature cultivation room, and general laboratory. The common feature of these rooms is the smooth and hard texture of the floors and walls, the simple purification level of the instruments and equipment, and the ease of cleaning.

Basic requirements for sterile laboratory:

preparation room

The preparation room is used for preparing culture media and sample processing. Indoor facilities include reagent cabinets, specialized case cabinets for storing equipment or materials, experimental benches, electric stoves, refrigerators, water supply and drainage systems, power sources, etc.

laundry room

The washing room is used for washing utensils, etc. Due to the contamination of used vessels by microorganisms, there may be pathogenic microorganisms present at times. Therefore, if conditions permit, it is best to set up a washing room. Indoor facilities should include heaters, pots, basins and buckets for washing utensils, as well as various bottle brushes, cleaning powders, soap, laundry detergent, etc.

Sterilization Room

The sterilization room is mainly used for sterilizing culture media and various utensils. The room should be equipped with sterilization equipment such as high-pressure steam sterilizers and ovens.

sterile room

A sterile room, also known as an inoculation room, is a specialized laboratory for sterile operations such as systematic inoculation and purification of bacterial strains. In microbial work, inoculation of bacterial strains

Transplantation is a major operation, and the characteristic of this operation is to ensure the purity of the bacterial strain and prevent contamination by miscellaneous bacteria. In the general air environment, due to the presence of many dust and miscellaneous bacteria, it is easy to cause pollution and greatly interfere with vaccination work.

Sterilization and disinfection of sterile rooms:

Fumigation: This is a measure of thorough sterilization in a sterile room. When the sterile room has been used for a long time and contamination is severe, fumigation sterilization should be carried out. Boroformaldehyde, lactic acid or sulfur fumigation.

Spray: before each use of the sterile room. Spray can promote the sedimentation of particles and microorganisms in the air, prevent the flying field of fine dust on the desktop and ground, and has a bactericidal effect. Can be sprayed with 5% carbolic acid

UV irradiation: performed before each use of the sterile room. Ultraviolet radiation has a good bactericidal effect. Usually, the ultraviolet lamp should be turned on for 30-60 minutes of irradiation.

Aseptic room working procedures:

Sterilization in sterile room

Turn on the ultraviolet lamp to irradiate for more than 30min before each use, or use 5% carbolic acid spray for internal and external rooms 30min before use.

Secondary sterilization

After washing hands with soap, move the necessary equipment into the outer room; Put on sterilized WORK clothes, work hats, and work shoes in the outer room, wear a mask, and then soak your hands in 2% coal phenol soap solution for 2 minutes.